BE/Bi 101: Order-of-Magnitude Biology
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چکیده
1. Entopy and viral packing. In the very first lecture, we learned how to do approximate integration by approximating curves as lines. We estimated how much energy it takes to pack the φ29 genome. As a reminder, the experimental setup is shown in Fig. 1a. A bead in an optical trap is connected to the φ29 DNA. The viral capsid is attached to an immobilized bead. The packing motor works to pack the genome into the capsid. The optical trap is calibrated such that the force counteracting packing can be measured. The resulting measurement is the force-packing curve in Fig. 1b. The area under this curve is the energy required to pack the ≈20,000 bp genome. 10.4. DNA PACKING: FROM VIRUSES TO EUKARYOTES 603
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BE/Bi 101: Order-of-Magnitude Biology
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